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Unformatted text preview: transplantation experiment) How did the authors correlate expression of CD34 and SCA1 with HSC? Do their experiments prove that the bone marrow cells in their pre-purified prep (Fr25, lin-, but-non homed) that express these proteins are HSC? Do the HSC mesodermal derived cells ever give rise to cells of ectodermal origin? of endodermal origin? (at least according to these authors!) How did these authors demonstrate that donor cells were adopting epithelial fates? How are epithelial cells recognized? How are the donor cells recognized? Which epithelial tissue had the most donor cells? What explanation do the authors provide for this Discuss how to culture an ES cell from mice. What about human ES cells? Can adult stem cells be cultured in vitro? (answ- yes, briefly but most are difficult to culture for a long period of time because they spontaneously differentiate)...
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- Spring '08