4- - indicate the figure that shows this can you outline a microarray experiment what is the probe how is the message processed to hybridize to the

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Takahashi highlights What is an iPS and why would they be useful? Describe how the authors make iPS cells. What factors do they use? why these 4? how did the authors choose these factors? What role do retroviruses play? What is a retrovirus? How are the original cells cultured? How is the transition to ES cell culture conditions made? What is an ES cell culture condition? The authors state that they require a high retroviral transduction efficiency to successfully make iPS cells. How was this achieved in human cells with the mouse retrovirus? Can you name / list at least 3 problems with the iPs cells described in this paper that would make them unsuitable for use in therapies? List all the different ways the authors demonstrate that the iPS cells are like ES cells and
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Unformatted text preview: indicate the figure that shows this. can you outline a microarray experiment? what is the probe? how is the message processed to hybridize to the probe? What information can you gather from Figure 2D about the transcription profile of iPS cells? why is MMLV RT particularly useful? how is doing a microarray experiment different from doing RT PCR? Can you outline a ChiP experiment? what is immunoprecipited? How do you make the DNA immunoprecipitate too? How do you detect the DNA that is co-precipitated? What is a teratoma? Can you describe what is meant by reprogramming? what approaches work to reprogram nuclei?...
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This note was uploaded on 10/19/2009 for the course B M B 460 taught by Professor Hanna-rose,w during the Spring '08 term at Pennsylvania State University, University Park.

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