2008 Exam 4 - BMB/Micrb 460 / May 5, 2008 NAME: Exam 4 /...

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BMB/Micrb 460 / May 5, 2008 NAME: Exam 4 / TEST FORM A 1 EACH QUESTION IS WORTH 4 POINTS UNLESS MARKED OTHERWISE 1. (3 pts) Which is the most common source of traditional embryonic stem cells? a. The one-celled, fertilized zygote b. The blastomeres of an 8-celled embryo c. The inner cell mass of the blastocyst d. Any germ layer: mesoderm, endoderm or ectoderm e. Undifferentiated cells of aborted fetuses 2. Which is the best formal proof that a mouse cell is pluripotent? a. When injected into a lethally irradiated mouse, the cell has long-term repopulation (LTR) ability. b. Under appropriate culture conditions, the cell is able to differentiate in vitro into at least one cell type from each germ layer. c. Upon injection into a blastocyst, the cell can contribute to every tissue of the resulting animal. d. It can be used for SCNT and give rise to a live birth e. We can only prove that a cell is multipotent, not that a cell is pluripotent. 3. What is the difference between a totipotent and a pluripotent cell and name the stage of development where pluripotentcy is likely lost. 4. (3 pts) Which of the following cell types could be considered determined but not fully differentiated? a. HSC b. Myoblast c. NSC (neural stem cell) d. All of the above e. None of the above 5. (3 pts) Choose from the following answers for the next three questions A. a clone B. a hybrid C. a chimera D. all of the above _____ transfer of a nucleus from an adult skin cell to an oocyte could yield: _____ injection of an embryonic stem cell into a blastocyst would most likely yield: _____ injection of an embryonic stem cell into a developing embryo, after formation of three germ layers, would most likely yield:
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Form A Name :_____________________________________ 2 6. If ES cells containing an engineered transgene were used to create the pigmented animal at the end of the schematic, the animal would a. have a 50% chance of being heterozygous for the transgene b. have a 50% chance of being homozygous for the transgene c. be homozygous for the transgene d. be heterozygous for the transgene e. be a mosaic animal with some cells heterozygous for the transgene and other cells homozygous for the transgene 7. Krauss et al used homing as a strategy to purify individual HSCs. Which feature of their experiment enabled them to actually isolate the desired cells after “homing” in the irradiated recipient mouse? a. They injected male, Y-chromosome containing marrow cells into female recipients b. They injected cells labeled with a fluorescent membrane dye called PKH26. c.
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2008 Exam 4 - BMB/Micrb 460 / May 5, 2008 NAME: Exam 4 /...

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