Unformatted text preview: What is FBS? Why was it used in the beginning and then removed later and replaced by ES medium? What does reseeding on a feeder mean? 6. The authors demonstrate in multiple ways that the iPS cells of HDF origin resemble ES cells. Can you list these, as well as the figure where they demonstrate the specific property? 7. If these were mouse iPS cells, what experiment could be used in place of all of the experiments listed in the question above? 8. What is the ultimate fate of the retroviral constructs in the cells? Are they integrated? Are they expressed in the short term? In the long term – which figure addresses long-term expression?...
View Full Document
This note was uploaded on 10/19/2009 for the course B M B 460 taught by Professor Hanna-rose,w during the Spring '08 term at Penn State.
- Spring '08