Takahashi guide

Takahashi guide - What is FBS Why was it used in the...

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Guide for Takahashi et al. (2007) Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors. Cell 131, 861–872. These questions are intended to help you read the paper in an inquisitive fashion and to help you focus on some of the issues you should understand. Please cover each of these questions during your reading and studying. 1. What is an iPS? 2. What is a retrovirus and why use a retrovirus rather than a plasmid to introduce the “stemness” genes into the cells? 3. Why does iPS formation require high virus transduction efficiencies? 4. How were higher efficiencies of retroviral transduction in adult fibroblasts achieved? 5. Figure 1A describes the culture conditions used during the experiment.
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Unformatted text preview: What is FBS? Why was it used in the beginning and then removed later and replaced by ES medium? What does reseeding on a feeder mean? 6. The authors demonstrate in multiple ways that the iPS cells of HDF origin resemble ES cells. Can you list these, as well as the figure where they demonstrate the specific property? 7. If these were mouse iPS cells, what experiment could be used in place of all of the experiments listed in the question above? 8. What is the ultimate fate of the retroviral constructs in the cells? Are they integrated? Are they expressed in the short term? In the long term – which figure addresses long-term expression?...
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This note was uploaded on 10/19/2009 for the course B M B 460 taught by Professor Hanna-rose,w during the Spring '08 term at Penn State.

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