Takahashi lecture notes

Takahashi lecture notes - What do you want to talk about in...

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1 What do you want to talk about in next class?
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2 Takahashi et al. (2007) Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors. Cell 131, 861–872. A. Major Questions •Can adult cells be reprogrammed to a pluripotent state? •Why would we want to do this / make iPS cells? •Why is pluripotency desirable? •Why is an “induced” pluripotent cell useful? B.Hypothesis •The same four factors that can induce a pluripotent state in adult mouse cells will induce a pluripotent state in adult human cells •What specific type of cell do they use? •What specific factors?
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C. Experimental Strategy: •Make cells express the 4 genes (Oct2/3, KLF4, cMyc, Sox2) •Retrovirus •Problems with this approach? •Transduction efficiency must be high - why? •How solved? Any potential problem here? •Place them in an environment permissive for ES cells •feeder •Test for ES cell character / pluripotency •~8 different assays
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Takahashi lecture notes - What do you want to talk about in...

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