This preview has intentionally blurred sections. Sign up to view the full version.View Full Document
Unformatted text preview: Guide for Takahashi et al. (2007) Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors. Cell 131, 861–872. These questions are intended to help you read the paper in an inquisitive fashion and to help you focus on some of the issues you should understand. Please cover each of these questions during your reading and studying. 1. What is an iPS? Induced pluripotent stem cell – in other words an adult cell of some type that has been made / forced to take on an embryonic stem cell character 2. What is a retrovirus and why use a retrovirus rather than a plasmid to introduce the “stemness” genes into the cells? Retroviruses are RNA viruses that enter the cell after binding to a receptor and then replicate via a DNA intermediate (the virus encodes its own reverse transcriptase). The DNA copy of the genome can be integrated into the host genome. This is likely desirable in terms of maintaining gene expression over a period of more than a couple of days, as cell division would dilute a plasmid vector. cell division would dilute a plasmid vector....
View Full Document
- Spring '08
- DNA, cells, Cellular differentiation, Embryonic stem cell