ExamformA_answers

ExamformA_answers - BMB/MICRB 460 / February 11, 2009 Exam...

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BMB/MICRB 460 / February 11, 2009 NAME: Exam 1 / TEST FORM A PLOT A PLOT B TEST DIRECTIONS: Choose a single answer for each multiple-choice question and RECORD it on the line to the left of the question. Write your answer to the short answer questions LEGIBLY in the space provided There are 25 questions, and each question is worth 4 points (unless noted). I will post the exam, the answers and your scores as soon as all students have taken the exam and grading is complete. 1. Hydropathy PLOT A was generated using the sequence of a protein that could not be extracted from the membrane pellet of lysed cells with high salt buffer but was extracted by TritonX-100. What hypothesis would you make about the nature and structure of the region of the protein that associates with the membrane? This is an intergral membrane protein with 2 transmembrane helical domains 2. PLOT B was generated using the sequence of a second protein with similar extraction properties. What hypothesis would you make about the nature and structure of this protein? This is also an intergral membrane protein (based on extraction properties). Since it has no transmembrane helical domains it must be embedded in the membrane via some other type of motif (accepted any answer such as lipid modified, palmitoylated, prenylated, GPI anchor or beta barrel transmembrane domain)
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BMB/MICRB 460 / February 11, 2009 NAME: Exam 1 / TEST FORM A A B A C D squirrel chip- munk 3. You plan to repeat the experiments of Frye and Edidin using some new cell lines: a squirrel-derived cell line and a chipmunk-derived cell line. You have as many animals available to you as you need of each of the following species for antibody production– rabbits, hamsters, and squirrels. Many answers possible here. The key here is to use TWO DIFFERENT species for answers A and B and then any species aside from the same one you used in A for C and any species aside from the same one used in B for D. I also did not give full credit for suggesting to inject squirrel cells into a squirrel unless you specifically pointed out about using lymph cells and spleen cells to do the injections and how this would result in only MHC antibodies –injecting squirrel cells into a squirrel is not the most “effective” way to produce antibodies – as was asked A.Which animal will you inject with the squirrel cell line to most effectively produce antibodies? hamster B. Which animal will you inject with the chipmunk cell line to most effectively produce antibodies? squirrel C. Choose an animal for production of a secondary antibody to the primary antibody produced in “A”. rabbit D. Choose an animal for production of a secondary antibody to the primary antibody produced in “B”. rabbit
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This note was uploaded on 10/19/2009 for the course B M B 460 taught by Professor Hanna-rose,w during the Spring '08 term at Penn State.

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ExamformA_answers - BMB/MICRB 460 / February 11, 2009 Exam...

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