Exam_2B_2009answers - BMB/MICRB 460 / March 5, 2009 NAME:...

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Unformatted text preview: BMB/MICRB 460 / March 5, 2009 NAME: Exam 2 / TEST FORM B TEST DIRECTIONS: Choose a single answer for each multiple-choice question and RECORD it on the line to the left of the question. Write your answer to the short answer questions LEGIBLY in the space provided There are 25 questions. Questions are worth 4 points unless stated otherwise. I will post the exam, answers and scores as soon as all students have taken the exam and grading is complete. 1. (5 points) The -adrenergic receptor stimulates adenylate cyclase via a trimeric G protein. Describe (or outline or diagram/draw) the sequence of events that begins with binding of adrenaline to the receptor and culminates with a change in adenylate cyclase activity. adrenaline binds receptor which undergoes conformational change receptor can now act as a GEF on G protein, causing alpha to let go of GDP and bind GTP GTP-bound alpha subunit can now activate adenylyl cyclase 2. (5 pts) Draw, diagram or describe the events that lead to activation of a receptor tyrosine kinase (RTK). Mention in your discussion: an example ligand, an example receptor, what happens upon ligand binding, and what residues become phosphorylated as well as any other event that is significant for activation of the receptor. (No need to discuss downstream events) EGF binds to receptor receptor dimerizes cytoplasmic domains phosphorylate themselves and their new neighbour phosphorylated tyrosines can now recruit downstream signaling proteins Form B NAME:_______________________________________ 2 3. (5 pts) Draw, diagram or describe a method to test if EGFR and PLC interact DIRECTLY. Include discussion of the source of your proteins for the experiment. lots of possible answers, key is two purified proteins that you mix together, then need to detect whether they have bound 4. Which of the following would result in a Ras protein that could not be switched to the on state? a. mutational inactivation of the GTPase domain of Ras- but can still bind GTP b. mutational inactivation of the effector domain of Ras c. mutational inactivation of the domain of Ras that binds SOS (a GEF) d. mutational inactivation of the domain of Ras that binds the GAP e. fusion to a transmembrane domain Form B NAME:_______________________________________ 3 5. The substrate of PLC is a. GTP b. IP3 c. DAG d. PIP2 e. ATP 6. Ras is a switch protein and cycles between an active and an inactive state in a manner similar to a. the alpha subunit of trimeric G proteins b. the gamma subunit of trimeric G proteins c. phospholipase C gamma (PLC ) d. the epidermal growth factor receptor (EGFR) 7. What type of enzymatic activity does Ras have?...
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This note was uploaded on 10/19/2009 for the course B M B 460 taught by Professor Hanna-rose,w during the Spring '08 term at Pennsylvania State University, University Park.

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Exam_2B_2009answers - BMB/MICRB 460 / March 5, 2009 NAME:...

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