Ex_4_09answers

Ex_4_09answers - BMB/MICRB 460 Exam 4 May 7 2007 TEST FORM...

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BMB/MICRB 460 / Exam 4 / May 7, 2007 / TEST FORM A NAME: 1 QUESTIONS ARE WORTH 3 POINTS UNLESS NOTED OTHERWISE 1. Compare and contrast the best experiment you can think of to investigate a) if a new mouse cell line has pluripotent cells and b) if a new human cell line has pluripotent cells (7 points). Best experiment for mouse: inject cells into blastocyst and make a chimeric mouse that demonstrates the injected cells can contribute to each tissue Best experiment for human – I would accept an in vitro differentiation strategy showing that the cells can become something from each of the ectodermal, endodermal and mesodermal lineages. Another experiment is to inject the cells into a an immunocompromised mouse and get a teratotoma that has cells from each germ layer (Terada paper did this) 2. Krause et al purified HSCs from bone marrow. Part of their procedure involved selecting Lin - cells. What does Lin - mean? And how did they select the Lin - cells? (6 points) Lin- means lineage depleted – or depleted of all terminally differentiated cells The select the Lin- cells by removing all the Lin+ cells. They use a mixture of antibodies that bind to cell surface markers on as many kinds of differentiated cells as they can (these surface markers are not on undifferentiated cells). They attached the antibodies to a plate and put the bone marrow cell mixture on the plates. Any terminal differentiated cells will stick to the antibodies and, thus, be bound to the plate. The other cells will not bind and are washed off the plate and kept.
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Name :_____________________________________ 2 3. The first determination event during mouse development occurs as a direct result of a. the first couple of rapid “early cleavages” b. compaction c. gastrulation d. neurotube formation e. implantation 4. Each cell in the 4 cell stage mouse embryo is generally considered to be a. totipotent b. pluripotent c. multipotent d. unipotent 5. HSCs are generally considered to be a. totipotent b. pluripotent c. multipotent d. unipotent 6. The stage of development where the dogma in the field of developmental biology states that pluripotentcy is probably lost is a. the first couple of rapid “early cleavages” b. compaction c. gastrulation d. neurotube formation e. implantation 7. (6 pts) Name the three germ layers and place these three cell types into the appropriate row: HSC, (NSC) Neural Stem Cell, intestinal epithelial cell LAYER CELL TYPE _______meso________________ ________HSC________________ _________ecto______________ _________NSC_______________ ________endo_______________ _________intestinal epithelial cell__
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Name :_____________________________________ 3 8. How were the cells in the peripheral blood at 5 and 11 months in Table 1 identified? a.
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This note was uploaded on 10/19/2009 for the course B M B 460 taught by Professor Hanna-rose,w during the Spring '08 term at Penn State.

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Ex_4_09answers - BMB/MICRB 460 Exam 4 May 7 2007 TEST FORM...

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