bis_104_pq_27_09 - (DAG) and phosphatidylinositol (PI), to...

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BIS 104 PQ 27 09 You are interested in studying the role of lipid composition in SNARE-mediated fusions. Using the in vitro fusion assay, another member of your lab previously observed that addition of the cone-shaped phosphatidic acid (PA), to 85 mol percent phosphatidylcholine (PC):15 mol percent phosphatidylserine (PS) acceptor liposomes at the expense of PS increases the rate of fusion; addition of inverted-cone shaped lyso-phosphatidic acid (LPA) was inhibitory. You are interested in testing the ability of two lipids found in biological membranes, diacylglycerol
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Unformatted text preview: (DAG) and phosphatidylinositol (PI), to either stimulate or inhibit fusion. 1) Given your labs previous findings, predict what the effects would be of including in acceptor liposomes either PI or DAG on SNARE-mediated fusion rates. 2) Please describe the experimental procedure you would use to test your predictions above. 3) What is/are your negative control(s) for this experiment? 4) What could serve as your positive control(s) for this experiment?...
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This note was uploaded on 10/20/2009 for the course BIS 104 taught by Professor Scholey during the Spring '08 term at UC Davis.

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bis_104_pq_27_09 - (DAG) and phosphatidylinositol (PI), to...

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