bis_104__techs_winter_09

bis_104__techs_winter_09 - Fluorescence Resonance Energy...

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BIS 104 winter 09 Check list of techniques that you should be able to (a) describe, and (b) use to answer questions about how cells function. Acceptable abbreviation are indicated in parentheses. Microscopy Light microscopy Bright field Phase-contrast and Differential Interference Contrast (DIC) Fluorescence scanning confocal deconvolution Electron microscopy Transmission EM (TEM) Scanning EM (SEM) Cryo-EM Other Atomic force microscopy (ATM) Immunohistochemistry (IHC) Direct immunofluorescence (direct IF) Indirect immunofluorescence (indirect IF) Fluorescence Recovery After Photobleaching (FRAP)
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Unformatted text preview: Fluorescence Resonance Energy Transfer (FRET) Fluorescence Activated Cell sorting (FACS) Single Particle Tracking (SPT) Pulse-chase labeling Autoradiography Cell fractionation Differential centrifugation Sedimentation velocity Equilibrium density Freeze-fracture Sodium dodecyl sulfate polyacrylamide electrophoresis (SDS-PAGE) Western blotting Northern blotting Reverse transcriptase polymerase chain reaction (RT-PCR) Cell transfection Cell-free translation system Fluorescent in situ hybridization (FISH) Cell culture...
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This note was uploaded on 10/20/2009 for the course BIS 104 taught by Professor Scholey during the Winter '08 term at UC Davis.

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