bis_104_pq_ldls_fall_08

Bis_104_pq_ldls_fall - OBSERVATION When exogenous LDLs are added to cells in culture the LDLs appear to bind to the plasma membrane in discrete

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
OBSERVATION : When exogenous LDLs are added to cells in culture the LDLs appear to bind to the plasma membrane in discrete clusters which are then internalized as clathrin coated pits. Hypothesis #1: Prior to exposure to LDLs, LDL receptors (LDL-Rs) are randomly and diffusely distributed around the cell’s PM. Binding of LDLs induces the lateral movement and clustering of the ligand-receptor complexes into clathrin coated pits prior to endocytosis. Hypothesis #2: Cells from individuals with the FH -/- mutation have normal amounts of LDL-Rs in their PMs, but they are incapable of lateral movement and fail to form clusters upon LDL binding. Design a series of experiments to test the plausibility and validity of these hypotheses. Expt a: Immunostain live cells from a normal individual with a fluorochrome tagged antibody against the external domain of the LDL-R. Apply this antibody to two batches of cells, before and after treating them with LDLs. Compare the fluorescent staining patterns by fluorescence
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 10/20/2009 for the course BIS 104 taught by Professor Scholey during the Winter '08 term at UC Davis.

Page1 / 2

Bis_104_pq_ldls_fall - OBSERVATION When exogenous LDLs are added to cells in culture the LDLs appear to bind to the plasma membrane in discrete

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online