BIS103_07MT2_ - BIS103-001(Winter 2007 Midterm#2(February...

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BIS103-001 (Winter 2007) Midterm #2 (February 27) Name_____________________________________ 1 Instructor: Abel Student ID #_______________________________ Pls., check appropriate box below. This exam consists of 6 questions. A maximum of 100 points can be earned. Partial credit will be given. There are a total of 12 pages, including the cover page and one blank sheet at the end for notes. However, do not use the blank sheet for your final answers. If you need more space, use the back of pages 2-11. Write your name on top of each page! Petitions for regarding will be considered only if you have used permanent ink , unless an addition error has occurred. *IT IS YOUR RESPONSIBILITY TO WRITE LEGIBLE! No extra effort will be made to decipher your handwriting. T = 25 o C (298 K) T = 37 o C (310 K) R = 8.315 J mol -1 K -1 F = 96.5 kJ mol -1 V -1 n = equivalents of electrons Z = charge of proton ! G o’ = – RTlnK eq ! G = ! G o ’ + RTln[Prod.]/[React.] ! G = ! G o ’ + 2.303RT ! pH + ZF !# ! G o’ = – nF ! E o’ ! E o’ = E o’ Oxidant – E o’ Reductant I,_______________________________________, authorize the University to distribute publicly this graded exam (e.g., handed out in class or left in a bin for pick up). I am aware of the fact that violations of the Academic Code of Conduct 1 may be reported to UC Davis Student Judicial Affairs. 1 Examples of academic misconduct include: receiving or providing unauthorized assistance on examinations, using unauthorized materials during an examination, altering an exam and submitting it for re-grading, or using false excuses to obtain extensions of time ( http://sja.ucdavis.edu/cac.htm ). Signature________________________________________ Date____________________________ Undergraduate Student Completing Incomplete Open Enrollment Student Graduate Student Question Value Score 1 21 2 21 3 12 4 25 5 7 6 14+2 TOTAL 100 (+2)
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BIS103-001 (Winter 2007) Midterm #2 (February 27) Name_____________________________________ 2 1. (21 pts) Questions on the ETC and proton gradient-dependent ATP synthesis. a) Some processes of the mitochondrial electron transport chain and of the “light reactions” in photo- synthesis are strikingly similar. Which components in chloroplasts and mitochondria are analogous ? Fill in the blanks. (6 pts) Component in Mitochondria Component in Chloroplasts 1. Inner membrane 1. _______________________________ 2. _____________________________ 2. Stroma 3. Intermembrane Space 3. _______________________________ 4. _____________________________ 4. Plastocyanin (PC) 5. Coenzyme Q 5. _______________________________ 6. _____________________________ 6. Cytochrome b 6 f complex b) Inhibition of the ATP/ADP translocator in the inner mitochondrial membrane of mice by gene knock- out technology causes reduced mitochondrial import of ADP in exchange for ATP. Metabolic consequences are the accumulation of NADH in the mitochondria and the accumulation of lactate in the blood of the “knock-out” mice. Provide a brief explanation for both observations. (6 pts)
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BIS103-001 (Winter 2007) Midterm #2 (February 27) Name_____________________________________ 3 c) An inhibitor is added to isolated mitochondria that are supplied with plenty of pyruvate and oxygen.
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