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Vijay_Week+3 - Click to edit Master subtitle style Biology...

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Click to edit Master subtitle style 10/21/09 Spore Stain, Plating, Aseptic culture, Pepper Infusion ii Biology 207. Introductory Microbiology Eric Elliott. 09/29/2009
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10/21/09 Recap from last two weeks Microscopy: Basic. Perfecting the art Smear preparation Simple staining Gram staining Observations: wet mount, heat-fixed Pepper infusion
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10/21/09 Tasks for today 1. Aseptic Technique and Transfer 2. Spread Plate with Milk 3. Environmental Contamination 4. Endospore Staining 5. Pepper Infusion II
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10/21/09 1. Aseptic Technique and Transfer Its all about the media. p 123-131 Consistency Purpose Sterilization Standard Operating Procedure. p 63 - 83 Broth to broth transfer: From broth culture inoculate ‘sterile’ broth using a sterile loop Broth to Agar plate: Take another loop full and streak plate. But how? …
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10/21/09 2. Spread Plate with Milk Use the metal spreader to streak milk Since the spreader is not sterile, dip it in alcohol, and flame dry. You need NOT heat till the spreader is red hot.,
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