Vijay_Week+3 - Click to edit Master subtitle style Biology...

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Click to edit Master subtitle style 10/21/09 Spore Stain, Plating, Aseptic culture, Pepper Infusion ii Biology 207. Introductory Microbiology Eric Elliott. 09/29/2009
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10/21/09 Recap from last two weeks Microscopy: Basic. Perfecting the art Smear preparation Simple staining Gram staining Observations: wet mount, heat-fixed Pepper infusion
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10/21/09 Tasks for today 1. Aseptic Technique and Transfer 2. Spread Plate with Milk 3. Environmental Contamination 4. Endospore Staining 5. Pepper Infusion II
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10/21/09 1. Aseptic Technique and Transfer Its all about the media. p 123-131 Consistency Purpose Sterilization Standard Operating Procedure. p 63 - 83 Broth to broth transfer: From broth culture inoculate ‘sterile’ broth using a sterile loop Broth to Agar plate: Take another loop
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10/21/09 2. Spread Plate with Milk Use the metal spreader to streak milk Since the spreader is not sterile, dip it in alcohol, and flame dry. You need
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Vijay_Week+3 - Click to edit Master subtitle style Biology...

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