Thin Layer Chromatography 2 - Pulliam/Mista 1 Thin Layer...

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Pulliam/Mista 1 Thin Layer Chromatography Joseph Mista Chris Pulliam Due: 9/15/09 Submitted: 9/15/09 __________________________________ ___________________________________
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Pulliam/Mista 2 PURPOSE : To use thin layer chromatography to check the purity of a compound STRUCTURAL FORMULA : Benzoic Acid: Ethyl 4-aminobenzoate: 9-Fluorenone: CALCULATIONS : Percent yield calculation: final amountinitial amountx100
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Pulliam/Mista 3 39mg acid50mg = % acidx100 78 Retention factor calculation: distance traveled by compounddistance traveled by solvent RESULTS : Compound Initial Amount Final Amount Melting Point o C Percent yield % Acid 50 mg 39 mg 89-92 78% Base 50 mg 35 mg 119-123 70% Neutral 50 mg .684 g 78-81 1368% The Retention factor values for each of the 5 TLC test are as follows (read from left to right across from TLC plates) RUN 1: 0.51, 0.54, 0.69, 0.69, 0.5 RUN 2 : 0.2, 0.3, 0.51, 0.53, 0.2 RUN 3: 0.2, 0.34, 0.54, 0.56, 0.21 RUN 4: 0.04, 0.07, 0.34, 0.33, 0.54, 0.74, 0.34, 0.03 ISOLATED TLC RUN: 0.14, 0.09, 0.11, 0.19 Run number 2 was the run that my group deemed to be the best. We came to this conclusion because the spots were not spread arbitrarily, they were a good distance from the bottom and the top, and were separated the best from the runs that were done. Run number two’s jar contained a diethyl ether to hexane mixture of 6 to 4, so that extra 2 mL of ether allowed the
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This note was uploaded on 10/21/2009 for the course MA 16200-018 taught by Professor Davidcatlin during the Spring '09 term at Purdue University-West Lafayette.

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Thin Layer Chromatography 2 - Pulliam/Mista 1 Thin Layer...

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