{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Thin Layer Chromatography 2

Thin Layer Chromatography 2 - Pulliam/Mista 1 Thin Layer...

Info icon This preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
Pulliam/Mista 1 Thin Layer Chromatography Joseph Mista Chris Pulliam Due: 9/15/09 Submitted: 9/15/09 __________________________________ ___________________________________
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Pulliam/Mista 2 PURPOSE : To use thin layer chromatography to check the purity of a compound STRUCTURAL FORMULA : Benzoic Acid: Ethyl 4-aminobenzoate: 9-Fluorenone: CALCULATIONS : Percent yield calculation: final amountinitial amountx100
Image of page 2
Pulliam/Mista 3 39mg acid50mg = % acidx100 78 Retention factor calculation: distance traveled by compounddistance traveled by solvent RESULTS : Compound Initial Amount Final Amount Melting Point o C Percent yield % Acid 50 mg 39 mg 89-92 78% Base 50 mg 35 mg 119-123 70% Neutral 50 mg .684 g 78-81 1368% The Retention factor values for each of the 5 TLC test are as follows (read from left to right across from TLC plates) RUN 1: 0.51, 0.54, 0.69, 0.69, 0.5 RUN 2 : 0.2, 0.3, 0.51, 0.53, 0.2 RUN 3: 0.2, 0.34, 0.54, 0.56, 0.21 RUN 4: 0.04, 0.07, 0.34, 0.33, 0.54, 0.74, 0.34, 0.03 ISOLATED TLC RUN: 0.14, 0.09, 0.11, 0.19 Run number 2 was the run that my group deemed to be the best. We came to this conclusion because the spots were not spread arbitrarily, they were a good distance from the bottom and the top, and were separated the best from the runs that were done. Run number two’s jar contained a diethyl ether to hexane mixture of 6 to 4, so that extra 2 mL of ether allowed the TLC plate to flow correctly and give us very accurate readings for determining our Retention factor values.
Image of page 3

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern