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Complementation Guidelines

Complementation Guidelines - Hey So for the problem 3's on...

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Hey, So for the problem 3's on the practice test, it has two major parts. The first is figuring out how many genes you have and which mutants are in which genes. This relies upon complementation. The second part is to figure out what the dominance/recessive relationships are for the different alleles within a gene. Complementation – Basic Rules - 1) All mutants must be recessive and true breeding for this to work. a) The line where all mutants are crossed to wildtype tells you if the mutants are recessive or dominant to wildtype. b) The diagonal where the mutants are crossed to themselves tells you if they are true breeding. 2) A complementation test is when you cross two mutants together and test the phenotype in the F1. There are two possibilities. a) If the mutants are in the same gene then the F1 phenotype will be a mutant phenotype and not wildtype. b) If the mutants are in different genes, then the F1 phenotype will be wildtype.
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