Practice Problem on Gene Expression Models

Practice Problem on Gene Expression Models - Stimulus A...

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Lecture Problem on Gene Expression - Given the above model of gene regulation address the following questions. You can chose any combination of the presence and or absence of Stimulus A and Z. 1) What happens to the output in the presence of a mutant in E (loss of function mutation) 2) What happens to the output in the presence of a mutant in C (loss of function mutation) 3) What happens to the output in the presence of a mutant in C (dominant mutation-B cannot repress C) 4) Cross eeCC x EEcc and what is outcome in F 2 a. Assume that the mutations act in trans . b. Assume that the mutation act in cis . Stimulus A Stimulus Z B C D E output 1
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The best first step is to figure out what happens to gene expression under the four possible combinations of stimuli. Lets say that the output is a purple color. Correspondingly, the lack of an output is white. There are four possible combinations of stimuli A) Both Stimulus A and Z – There is no color in this situation. For this example, I’ve greyed in the points that are repressed and thereby absent. As you can see, stimulus A leads to the shutting down of C/D and leads to no purple. Likewise, stimulus Z also shuts down the output by inducing E which represses the output. B) Only Stimulus A is present – There is no color/output in this situation. Stimulus A shuts down the activators C and D. C) Only Stimulus B is present – There is no color/output in this situation. C and D are active but Stimulus Z causes E to repress the output. D) Neither Stimulus A nor B is present – There is color/output in this situation. The lack of
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This note was uploaded on 10/21/2009 for the course BIS 52192 taught by Professor Enochbaldwin during the Fall '09 term at UC Davis.

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Practice Problem on Gene Expression Models - Stimulus A...

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