{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

MCDB101Bslideset4

MCDB101Bslideset4 - 133 Chromosomes(Chpt 13 Chromatin DNA...

Info iconThis preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon
1 Chromosomes (Chpt. 13) Chromatin – DNA, histone proteins, nonhistone proteins – Single DNA strand per chromatid Histones – 5 basic proteins – Nonhistones – heterogeneous collection of 1000s of different proteins Nucleosome – fundamental unit of chromosomal packing Tight association of DNA and protein octomer core consisting of 2 @ H2A, H2B, H3, H4 ~160 bases around core with ~40 bases of linker DNA before next nucleosome – H1 histone interacts with DNA outside the core octomer 133 Co In E Nonhistone Proteins 13.2 Nuclear Scaffold Kinetochore Protein (CENP-E) 134 Co In E
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
2 13.3 135 Co In E 13.4 136 Co In E
Background image of page 2
3 Radial Loop-scaffold model – experimental evidence 13.5 EM of metaphase chromosome with histones extracted DNA is spread on flat surface – only loops observed 137 Co In E Chromosome Banding 13.6 Banding patterns are highly reproducible. Intrinsic property of each specific chromosome region. Must be determined by the DNA sequence. 138 Co In E
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
4 Specialized chromosomal elements ensure accurate replication and segregation of chromosomes There are many origins of replication on @ chromosome Replication occurs in about 8 hours during S phase in actively dividing human cells DNA polymerase can assemble new DNA at a rate of about 50 nucleotides per second In mammals, there are 10,000 origins of replication Each bidirectional replication is called a replicon
Background image of page 4
Image of page 5
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}