M101A_2008_Exam3key - MCDB 101A Summer 2008 Exam 3(100...

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1 MCDB 101A Summer 2008 Exam 3 Name__________ Key______________ _______________ 7/31/08 (100 points total) Perm #__________________________________________ Circle the best answer for each of the multiple choice questions (3 points each) 1. Which of the following processes requires RecA? a. Formation of a cointegrant during replicative transposition b. Formation of an Hfr strain c. Formation of a specialized transducing phage d. Formation of an F prime strain e. b, c and d 2. The primary factor that determines if it is possible to measure the cotransduction frequency of two genetic markers is a. the number of homologous regions located between two markers b. the number of inverted repeats between the markers c. the presence of att sites within the phage DNA d. whether the markers are associated with wild type or mutant alleles e. the maximum length of DNA that can be packaged within the bacteriophage particle 3. Which process plays the most significant role in the formation of multi-drug resistant plasmids? a. recombination b. transposition c. cotransduction d. replication e. conjugation 4. Which of the following is true of the ordered clone method of genomic sequencing? a. Genome is fragmented into small (~1kb) pieces b. All cloned fragments are sequenced c. Involves restriction enzyme mapping (i.e. physical mapping) of the entire genome d. Is not as effective as the shotgun method for sequencing repeated DNA sequences e. Was method used to sequence the E. coli genome 5. Recombination events between donor and recipient DNA during a transduction event will fail to yield viable cells if a. there are an even number of crossover events b. there are an odd number of crossover events c. there are more than two crossovers d. E. coli genomic DNA is transferred during transduction e. The donor DNA contains pseudo-pac sites 6. Which is true of an Hfr strain? a. Its F factor DNA exists in the form of a plasmid b. It is able to transfer genomic DNA to an F- recipient cell less efficiently than an F+ strain c. It is unable to undergo conjugation d. It is produced by the integration of an F plasmid into the E. coli genome e. It is produced by excision of a prophage from genomic DNA
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This note was uploaded on 10/22/2009 for the course MCDB 101A taught by Professor Thrower during the Summer '08 term at UCSB.

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M101A_2008_Exam3key - MCDB 101A Summer 2008 Exam 3(100...

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