M101A_2008Exam2key

M101A_2008Exam2key - MCDB 101A Summer 2008 Exam 2 7/21/08...

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1 MCDB 101A Summer 2008 Exam 2 Name____ Key__________________ _________________ 7/21/08 (100 points total) Perm #__________________________________________ Circle the best answer for each of the multiple choice questions (3 points each) 1. Which of the following evidence supports the Holliday model? a. Mismatches that are generated as a consequence of recombination b. A requirement for DNA polymerase I c. Recombination events occurring near Chi sites d. Recombination events associated with gene conversion e. both b and c 2. In the absence of inducer, a merodiploid of genotype I -d P + O + Z + Y-/I + P-O C Z-Y + will produce a. Both β galactosidase and permease b. β galactosidase but no permease c. Permease but no β galactosidase d. Neither β galactosidase nor permease e. Blue colonies on X-gal media 3. The presence of short nucleotide repeats or exposure of DNA to intercalating agents can produce a. apurinic sites b. nucleotide insertions/deletions c. frameshift mutations d. inversions e. both b and c 4. Which of the following is not included in a polymerase chain reaction mixture? a. A DNA template b. A pair of DNA primers c. A heat-stable polymerase d. The 4 deoxynucleotides e. DNA ligase 5. Which of the following is true of the SOS system? a. Is activated by the binding of RecA to double-stranded DNA b. Is responsible for the cleavage of LexA and CI c. Promotes the repair of pyrimidine dimers d. Is activated by ionizing radiation e. Both a and d are true 6. A mutation that changes an amino acid to another amino acid without creating an alteration in protein function is known as a a. silent mutation b. neutral mutation c. missense mutation d. nonsense mutation e. redundant mutation
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2 7. Which of the following is true of the blue-white cloning assay? a. It is based on α complementation b. Insertion of a gene into a blue-white cloning vector is detected by the appearance of blue colonies c. It involves recombination d. Requires that a wild type β -galactosidase gene is present in the bacterial genome e. None of the above statements are correct 8. Phage lambda CII protein is most similar in function to which of the following proteins? a. CAP
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M101A_2008Exam2key - MCDB 101A Summer 2008 Exam 2 7/21/08...

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