101A_Su09_midterm1key

101A_Su09_midterm1key - MCDB 101A S09 Midterm 1(100 points...

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MCDB 101A S09 Midterm 1 Name____ Key__________________ _________________ 7/6/09 (100 points total) Perm #__________________________________________ Multiple Choice: Circle the letter corresponding to the best answer (3 points for each correct answer) 1. If a DNA molecule consists of 20% A, 30% T and 20% G, which of the following is correct? a. The C content of the molecule must be 30% b. The C content of the molecule must be less than 30% c. The C content of the molecule cannot be determined from the information given d. The molecule is not double stranded e. Both a and d are correct 2. Restriction endonucleases and topoisomerase II share which of these features in common? a . They cut both strands of a double-stranded DNA molecule b. They are able to rejoin the ends of a broken double-stranded DNA molecule c. They can alter the number of supercoils in a closed circular DNA molecule d. They create single-stranded nicks in a DNA molecule while leaving the opposite strand intact e. both a and c are correct 3 . Which of the following is an activity directly associated with RNA polymerase but not DNA polymerases? a. 5’ to 3’ polymerase activity b. 3’ to 5’ exonuclease activity c. Helicase activity d. Gyrase activity e. Primase activity 4. Which of the following information can be obtained from the trans test? a. The relative order of mutations on a chromosome b. The relative distance between mutations c. Whether mutations are in the same or different genes d. Whether mutations are dominant or recessive e. Both a and b 5. Which of the following is not a conditional mutation? a. A mutation that makes a bacterial cell more sensitive than a wild type cell to cold b. A mutation that blocks the transport of an essential metabolite into a cell c. A mutation that blocks the synthesis of an essential metabolite d. A bacteriophage host range mutation e. Neither b nor c are conditional mutations 6. You carry out a cis test between 2 bacteriophage having genotypes AbCd and aBcD. Assuming you have assays that allow you to distinguish between all possible phenotypes associated with these markers how many different phenotypes would you expect to see? a. 4
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This note was uploaded on 10/22/2009 for the course MCDB 101A taught by Professor Thrower during the Summer '08 term at UCSB.

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101A_Su09_midterm1key - MCDB 101A S09 Midterm 1(100 points...

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