101A_Su09_finalkey

101A_Su09_finalkey - MCDB 101A Final Exam 200 points total...

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1 MCDB 101A Final Exam Name______ KEY _____________________________ July 31, 2009 200 points total Perm#_______________________________________ Choose the best answer to each of the following multiple choice questions (3 points each) 1. The Chi sequence is GCTGGTGG. What is the probability of finding this exact series of nucleotides within a random DNA sequence? a. 1/(4) 8 b. 1/(8) 4 c. 4/(8) 4 d. 1/(2) 8 e. 1/(8) 2 2. Hemimethylation of two DNA strands is an important feature of a. transcriptional activation b. transcriptional termination c. mismatch repair d. replication-coupled transposition e. both c and d 3. What specific conclusion can be drawn from Luria and Delbruck’s fluctuation experiment? a. Mutations occur randomly throughout the T1 phage genome b. Mutations occur randomly throughout the E. coli genome c. Mutations occur randomly over time within a dividing population of E. coli d. The formation of most mutations requires DNA replication e. Mutations occur more frequently in individual cultures than in large cultures 4. A RuvC loss of function mutation would block recombination at which of these steps? a. Two unpaired DNA molecules b. Two paired DNA molecules, at least one of which contains a nicked strand c. Two paired DNA molecules joined in a Holliday junction that have not undergone strand migration d. Two paired DNA molecules joined in a Holliday junction that have completed strand migration e. Two paired DNA molecules, each of which contains a nicked strand 5. Cotransduction frequencies for the genes val, pro, and gly were determined as follows: pro-gly = 0.1, pro-val = 0.6, val-gly = 0.5. Which of the following is true? a. Gene order is pro-val-gly and val is closer to gly than it is to pro b. Gene order is val-pro-gly and pro is closer to gly than it is to val c. Gene order is pro - val-gly and val is closer to pro than it is to gly d. Gene order is pro-val-gly and pro is closer to gly than it is to val e. Gene order is pro-gly-val and gly is closer to val than it is to pro 6. Which of the following differentiates generalized transduction from specialized transduction? a. Generalized transduction can potentially transfer any gene from one host cell to another, while specialized transduction can only transfer a limited subset of genes. b. Generalized transduction is carried out by phage that have both a lytic and lysogenic cycle while phage that carry out specialized transduction have only a lytic cycle. c. Specialized transduction requires a specialized structure known as a pilus d. F+ strains carry out generalized transduction while HFR strains do specialized transduction. e. Specialized transduction occurs through homologous recombination while generalized transduction requires site-specific recombination.
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2 7. Streptomycin is added to selective media in conjugation-based gene mapping in order to a. select for retention of the F plasmid b. eliminate the donor cells following the conjugation reaction c. eliminate the recipient cells following the conjugation reaction d. eliminate both donor and recipient cells following the conjugation reaction e. eliminate streptomycin resistant bacteria that have become resistant due to spontaneous mutations
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101A_Su09_finalkey - MCDB 101A Final Exam 200 points total...

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