Transc_Transl

Transc_Transl - mRNA Transcription and Translation Colinear...

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1 1 Transcription and Translation 2 mRNA • Colinear with gene sequence • Codes for one or more proteins • One DNA strand serves as coding strand for a given mRNA (has same sequence as mRNA) • Other strand is template strand (has a sequence complementary to that of the corresponding mRNA) • mRNA is synthesized 5'-3' 3 Both DNA Strands Can Serve as a Template for mRNA Griffiths Fig 10-6 Trun Fig 12.2 4 Genes Consist of Regulatory and Coding Sequences Fig 5.3
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2 5 RNA Polymerase • Core enzyme: β , β ' α 2 • Holoenzyme: Core + sigma factor ( σ ) • Sigma factor binds to promoter and localizes core enzyme to transcription initiation site Trun Fig 12.3a 6 Trun Fig 12-3 Promoters are DNA Sequences Where the RNA Polymerase Holoenzyme Binds to Initiate Transcription Pribnow box 7 E. coli Promoter Consensus Sequences Griffiths Fig 10.8 8 Correct Spacing Between The Pribnow and -35 box is Required for Polymerase Binding Fig 3.3 Molecular Genetics of Bacteria by Dale & Park
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3 9 Transcription A . Initiation – 1. RNA polymerase binds to promoter ( closed configuration ) – 2. Polymerase unwinds DNA helix ( open configuration
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Transc_Transl - mRNA Transcription and Translation Colinear...

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