Replication

Replication - DNA Replication "It has not escaped our...

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1 1 DNA Replication “It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material” -Watson and Crick 2 DNA Replication Models Fig 3.1 3 Meselson and Stahl Experiment •G row E. coli for many generations in 15 N media • Transfer to 14 N for successive generations • Following each round of replication, separate DNA with cesium chloride gradient • Determine whether DNA molecules are heavy, light or hybrid 4
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2 5 Griffiths Fig 8-13 (See Russell F. 3.1 6 Replication in E. coli is Bidirectional Rodriguez Griffiths Fig. 8-18 (See Russell Fig 3.9) Replication Forks 7 DNA Synthesis Runs 5’ to 3’ 5’ P OH 3’ 5’ P OH 3’ 8 Major Replication Genes /Proteins DNA initiator complex -denatures strands, opens replication bubble dnaB /DNA Helicase – extends replication forks gyrA B /DNA Gyrase -relieves supercoiling ahead of replication fork. Gyrase is a type II topoisomerase
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Replication - DNA Replication "It has not escaped our...

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