DNA - DNA: The Genetic Material Griffith and Bacterial...

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1 1 DNA: The Genetic Material 2 Griffith and Bacterial Transformation Streptococcus pneumoniae polysaccharide capsule = virulence factor • S-strain (encapsulated)-lethal to mice • R-strain-nonpathogenic 3 Fig. 2.2 The Griffith Mixed Culture Experiment 4 Nature of the Transforming Material: Avery, MacLeod, and McCarty 1. Heat kill S-strain 2. Detergent lyse bacteria and separate cytosol from cellular debris 3. Treat cell extract with enzymes to degrade proteins, lipids and carbohydrates 4. Mix enzyme treated cytosol with R-strain and examine colony morphology
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2 5 Fig 2.3 6 The Hershey-Chase Experiment • Bacteriophage T2 • Label phage with either 35 S or 32 P • Mix phage with E. coli • Separate phage from bacteria with a Waring blender • Pellet bacteria in centrifuge • Determine how much radioactivity associated with each isotope is in pellet vs. supernatant 7 Fig 2.6a 8 Fig 2.6b
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3 9 Fig 2.7 Deoxyribose Sugars differ from Ribose Sugars by the absence of a hydroxyl group at the 2’ position
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This note was uploaded on 10/22/2009 for the course MCDB 101A taught by Professor Thrower during the Summer '08 term at UCSB.

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DNA - DNA: The Genetic Material Griffith and Bacterial...

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