Lecture7 - Some more on TLC X A R f = A X Solvent front...

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Unformatted text preview: Some more on TLC X A R f = A X Solvent front Start Recall that the R f is not a physical constant. It depends on all of the following: 1. The amount of compound spotted. 2. The stationary phase used. 3. The mobile phase used. 4. The temperature. Assuming that the same solvent system and plates are used: •If the R f values from 2 plates are very different, then the compounds are different. •If the R f values from 2 plates are similar but not identical AND all visualization information is identical, then the compounds might be the same or different. Why? There are potential errors associated with R f values: Accuracy of the solvent front. How was the middle of the spot deFned. Assuming that the same solvent system and plates are used: •If the R f values from 2 plates are very different, then the compounds are different. •If the R f values from 2 plates are similar but not identical AND all visualization information is identical, then the compounds might be the same or different. Why? There are potential errors associated with R f values: Accuracy of the solvent front. How was the middle of the spot deFned. How much compound was spotted. Assuming that the same solvent system and plates are used (continued): •If the R f values from 2 plates are identical AND all visualization information is identical, then the compounds might be the same or...
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Lecture7 - Some more on TLC X A R f = A X Solvent front...

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