Lecture 8 - Problems of DNA Synthesis Unwinding Tension...

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Nucleotide – Nucleoside with phosphate added
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The DNA Double Helix DNA structure Double helical major, minor grooves right-handed bases are 3.4 Å apart (10 Å = 1 nm) 10 bases/turn Complementary Base Pairing through H bonds: A=T, G C Antiparallel Strands 5’ to 3’ 3’ to 5’
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Base-Pairing in DNA A=T G C
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Directionality of DNA synthesis
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Reading DNA Strands Single strand of DNA: 5’-AGCATTCG-3’ 3’-TCGTAAGC-5’ Complementary strand of above, usually written 5’ to 3’: 5’-CGAATGCT-3’ Double-stranded fragment is written: 5’-AGCATTCG-3’ 3’-TCGTAAGC-5’
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DNA Replication is Semiconservative
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DNA polymerases I and III pol I most abundant (400/cell) RNA primer removal pol III low abundance (15/cell) DNA replication
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Unformatted text preview: Problems of DNA Synthesis Unwinding Tension must be relieved Priming Antiparallel strands RNA primer removal Backbone joining Proofreading Initiation of Synthesis RNA Primase makes RNA primer on DNA template DNA Polymerase III extends primer with DNA DNA Polymerase I removes RNA primer, replaces with DNA Continuous and Discontinuous Synthesis Continuous on Leading Strand. Discontinuous on Lagging Strand creates Okazaki fragments. DNA ligase joins nicks in backbone. DNA Polymerase Activity 'replication fork' moves along DNA leading strand lagging strand numerous replication forks act simultaneously...
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Lecture 8 - Problems of DNA Synthesis Unwinding Tension...

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