Oct. 10, 2008 six per page

Oct. 10, 2008 six per page - Study Resources EXAM READER 1...

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1 Lab Exam 1: Th 10/16 6:40 -8:20 Study Resources: EXAM READER 1) Previous lectures (webcast) 2) Reviews- Friday 10/3 8-10 PM, Webcast! Friday 10/10 6-8 PM. Webcast? Monday 10/13 5-6:30 PM. 3) Practice problems 4) Punnett squares 5) Study resources (lab exam 1). Controls • Why? • What would they include and why? Pre-labs and worksheet answers posted outside 2084 VLSB. Monday 10/13 Lecture Review; afterwards several UGSIs to answer questions in 1 Pimentel. Office Hours: M 11-1 Mike, 1-2 Lingyan, 2-3 Thomas, 3-4 Iris. T 8:30-11 Aaron, 11:30-1 Helen, 1-2 Thomas/Oriana, 2-4 Mike, 4-5 Iris, 5:30-6:30 Gary. W 1-2 Mike/Lingyan, 2-3 Mike, 4-5 Iris, 5:30-6:30 Jeung. Th 8:30-10 Gary, 10-12 Mike, 1-2 Christian/Mike 5:30-6:30 CANCELED F 1-2 CANCELED Resolution: Agarose gel Standard curve Distance migrated (mm) # of bp (semi-log) λ DNA Hind III digest Detection methods - EtBr, radioactivity-- # of molecules. + - Migration is dependent upon shape! L R SC Detection methods - EtBr, radioactivity-- # of molecules.
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2 1N, 1C 1N, 1C 2N, 2C 2N, 4C Campbell 13.5 Campbell 13.3 Ploidy -sets of chromosomes (N vs. C) Genes & location (locus) & mutation Loss of function Mutants: single mutation, recessive Info from Mom Info from Dad Complementation: Key concepts Yeast life cycle-1N & 2N Mitosis Mitosis Fertilization Meiosis a’ b’ c’ d’ e’ f’ g’ h’ Biosynthetic pathways Analysis in a diploid. c’ d’ f’ Analysis in a haploid. 2 sets 1 set c’ d f’ Histidine (A.A). Remember this enzyme is encoded by a gene. Defective enzyme = mutant gene. Rescue by the addition of a chemical? Rescue by the addition of a good gene? - Mating CHEMICALS DO NOT RESCUE Complementation: Pathway CHEMICALS COULD RESCUE Complementation: Key concepts Mating- generating diploids a’ b’ e’ f’ g’ h’ Histidine (A.A). c’ d’ K1 a’ b’ e’ f’ g’ h’ Histidine (A.A). c’ d’ M1 a’ b’ e’ f’ g’ h’ Histidine (A.A). c’ d’ K1 a’ b’ e’ f’ g’ h’ Histidine (A.A). c’ d’ M2 YPD plate SD plate NO Histidine Replica plate Time: Growth? Cross 1 Cross 2
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3 Complementation i) Scoring growth!
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Oct. 10, 2008 six per page - Study Resources EXAM READER 1...

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