lectures 10-21 and 10-23

Lectures 10-21 and - Making a Gene Knockout in the Mouse Conservative Site-Specific Recombination Involves recombination between two defined

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Making a Gene Knockout in the Mouse
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Conservative Site-Specific Recombination Involves recombination between two defined sequence elements. A recombinase enzyme is required. A single crossover between a circular DNA molecule and a target DNA, results in insertion of the circular molecule into the other DNA.
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Site-Specific Recombination The recombination elements have directionality (polarity). The recombination sites must be aligned with the same polarity for recombination. The topology of the recombining DNA molecules and the orientation of the recombination sequence elements determine the outcome of the recombination event.
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The Recombinase Target Site Recombinase proteins typically bind as tetramers, one for each sequence site (two on each DNA molecule). The recombinase brings together the four recognition sequences, bringing together the two DNA molecules. The recombinase catalyzes cleavage of the
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This note was uploaded on 10/29/2009 for the course CHEM 105B at USC.

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Lectures 10-21 and - Making a Gene Knockout in the Mouse Conservative Site-Specific Recombination Involves recombination between two defined

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