lab 5 analysis - Pinkey Rahman (pr5989) Bio 205L Lab 5:...

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Bio 205L Lab 5: Eukaryotic Cell Analysis 1) There were six stages observed during the sea urchin development. The first stage was the fertilization in which the sperm fertilizes the sea urchin egg by acrosome reaction. The egg expanded slightly, growing from 100 micrometers to 125 micrometers. Also polyspermy is prevented by developing a envelope surrounding the egg. Next, the cell undergoes mitosis and split the cells into two cells. After about 20 minutes the cell underwent mitosis again, forming a 4-cell. Within the next 40 or 60 minutes the cell continued to divide until a blastula is formed, which looks like a hollow ball. Then eventually gastrulation occurred and lead to the development of endoderm, mesoderm and ectoderm germ layers. The embryonic cell is slightly larger than the original gamete cell. Once the cell undergoes mitosis and split, the surface area of the cell greatly increases with each stage while the overall volume of the cell remains the same. 2) Calculated sperm= Average number of sperm per large box/ 0.0001ml Sample # Dilution Average number of sperm per large box Calculated Sperm/ml Tube 1 1:100 5050 5.50x10^ 7 Tube 2 1:200 3570 3.57x10^ 7 Tube 3 1:400 2250 2.25x10^ 7 Tube 4 1:800 1125 1.125x10^
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lab 5 analysis - Pinkey Rahman (pr5989) Bio 205L Lab 5:...

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