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lab 6 analysis - PInkey Rahman(pr5989 Bio 205 L T.A Brad...

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PInkey Rahman (pr5989) Bio 205 L T.A- Brad Reveal Lab 6: Chloroplast Isolation 1) Chlorophyll concentration in 80% acetone solution Mg Chl/ml = {(0.532)(.020)} + {(0.812)(0.0080)} = 0.01714 mg Chl/ml 2) Chlorophyll concentration in homogenizer containing isolated spinach chloroplasts C1 x V1 = C2 x V2 C1 x 0.1 ml = 0.01714 mg Chl/ml x 10ml C1 = 1.714 mg Chl/ml 3) A) Mg Chl a/ml = {(0.812)(0.013)}-{(0.532)(0.0027)} = 0.00668 mg Chl a/ml B) Mg Chl b/mL = {( 0.532)(0.023)} – {(0.812)(0.0047)} = 0.00842 mg Chl b/mL 4) Mg Chl b/mL = { mg total Chl/ml } – {mg Chl a/mL} = (0.01714 mg Chl/ml) – ( 0.00668 mg Chl a/ml)
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= 0.01046 mg Chl b/mL The two values for mg Chl b/mL differ by 0.00204 mg Chl b/mL. This was probably due to experimental error in measuring the wavelengths of the chlorophyll. 5) [ mg Chl a] / [mg Chl b] = (0.00668 mg Chl a /ml) / (0.00842 mg Chl b/mL) = 0.793 6) Height of solvent front form the origin = 77mm Pigment Height (mm) R f Beta-carotene 60 (60/77)mm=0.779 Chlorophyll a 54 (54/77)mm=0.701 Chlorophyll b 50 (50/77)mm=0.650 Chlorophyll b has the most polarity and the least soluble since it traveled the shortest
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