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Unformatted text preview: (Ans: -7.1 x 10-9 m 2 /V s and -9.3 x 10-9 m 2 / V s, respectively) From this information can you explain why Protein B elutes after Protein A? B Protein for s kV / m 10 x 9.3-s kV / m 10 x 6.98-s kV / m 10 x 05 . 6-so A Protein for s kV / m 10 x 7.2-s kV / m 10 x 6.98-s kV / m 10 x 26 . 6-so 2 6-ep 2 5-2 5-eo app ep ep eo app 2 6-ep 2-5 2-5 eo app ep ep eo app = μ = μ μ = μ μ + μ = μ = μ = μ μ = μ μ + μ = μ The negative sign indicates that the proteins are negatively charged and are thus trying to electrophoretically move towards the anode (positive electrode). Protein B has the larger electrophoretic mobility so it must be more negatively charged then Protein A. If Protein B has a greater negative charge it can more strongly resist the pull of the electroosmotic flow as compared to Protein A, and thus comes off later....
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This note was uploaded on 11/03/2009 for the course CHEM 241 taught by Professor Tiani during the Summer '08 term at UNC.
- Summer '08