Chromatography_Problem_Set_2_Key

Chromatography_Problem_Set_2_Key - Chromatography Problem...

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Chromatography Problem Set 2: Key 1. Explain why in liquid chromatography it is not feasible to use an open tubular column, but it is feasible in gas chromatography? Diffusion in liquids is approximately 100 times slower than diffusion in gas. Consequently, the time it would take a solute molecule traveling down the center of a column to diffuse to the stationary phase coated on the inside wall of an open tubular column would be very long compared to the time it would take a solute molecule to reach a packing material particle in a packed column. 2. If you were to use a wall-coated open tubular column in an LC experiment, what would be the van Deemter equation? Using an open tubular column so no A term. H = B/u x + Cu x 3. If you were to use a wall-coated open tubular column in an LC experiment, which term in the van Deemter equation would be most adversely affected? The mass transport term (Cu x ). 4.
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Chromatography_Problem_Set_2_Key - Chromatography Problem...

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