Chapter_23_092205_Instructor

Chapter_23_092205_Instructor - FiveTypesofChromatography...

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 Five Types of Chromatography   Affinity Chromatography Adsorption Chromatography Partition Chromatography Ion-Exchange Chromatography Size Exclusion Chromatography
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 The Chromatogram   Detector Response Time (sec) t r1 t r2 t r3
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' 1 ' 2 1 2 1 r 2 r k k K K ' t ' t = = = α t r2 > t r1 α > 1.0 → You have a separation!!!  Measures of Retention  Selectivity Coefficient (α) • If you “α” then you the separation between two components. Differential Migration
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t – t ≡ time solute spends in stationary phase  Measures of Retention  Capacity Factor (k’) m s m m r ' n n t t - t k = =
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C ≡ Concentration of solute in stationary phase.  Measures of Retention  Capacity Factor (k’) m s m m r ' n n t t - t k = = = = m s m m s S V V K V C V C aq org M S [A] [A] C C K =
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 Fraction of Solute in Mobile Phase  Think about Solvent/Solvent Extraction → How much solute was extracted or was left behind. s
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This note was uploaded on 11/03/2009 for the course CHEM 241 taught by Professor Tiani during the Summer '08 term at UNC.

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Chapter_23_092205_Instructor - FiveTypesofChromatography...

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