Chapter_23_Part_II_Lecture_Notes

Chapter_23_Part_II_Lecture_Notes - Rate = B. Modified...

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Chapter 23: Fundamentals of Chromatography I.  Introduction to Chromatography A.   What is chromatography and how does it differ from solvent-solvent                    extraction? B.   Five Types or Modes of Chromatography: 1. 2. 3. 4. 5. C. What do all five types of chromatography have in common?
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II.  Fundamental Chromatographic Terms A. Flow Rate 1. Volume flow rate Consider a column with an inner diameter (i.d.) of 0.60 cm (r = 0.30 cm) and the mobile phase occupies 20 % of the column. Each centimeter of column length has a volume: V = (πr 2 x length) = π(0.30 cm) 2 (1cm) = 0.283 cm 3 or 0.283 mL 20 % of this 0.283 mL volume is mobile phase: 0.20 x 0.283 mL = 0.0565 mL Time (sec) t r1 t r2 t r3
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2. Linear flow rate B. The Chromatogram C. Retention times 1. t m 2. t r 3. t r III. Measures of Retention A. Relative Retention (α) B. Capacity Factor (k’)
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IV. Fraction of Time Solute (Analyte) Spends in the Mobile Phase in the Column V.  Rate of Solute Travel through Column. A.  
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Unformatted text preview: Rate = B. Modified Retention Time Expression: t = t + K V V r m s m 1 C. Modified Retention Volume Expression: VI. Efficiency of Separation A. Chromatographic Peak Shape B. Why do chromatographic peaks typically take on a Gaussian shape? V = V + K V V r m s m 1 C. Resolution (R) D. Plate Height and Plate Theory 1. What is Plate Theory? (A) C M C S (B) C M C S 2. How is the Plate Height a Measure of Column Efficiency? 3. Determining the Number of Plates 4. Resolution Revisited VII. Band Broadening A. Van Deemter Equation B. Terms within the Van Deemter Equation 1. Multiple Paths Term 2. Longitudinal Diffusion Term 3. Mass Transport Term C. Van Deemter Plot VIII. Asymmetric Bandshapes A. Overloading B. Tailing...
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This note was uploaded on 11/03/2009 for the course CHEM 241 taught by Professor Tiani during the Summer '08 term at UNC.

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Chapter_23_Part_II_Lecture_Notes - Rate = B. Modified...

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