Chapter_25_HPLC_Lecture_Notes

Chapter_25_HPLC_Lecture_Notes - 1. Adsorption...

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Chapter 25: High Performance Liquid Chromatography (HPLC) I.  High-Performance Liquid Chromatography (HPLC) A.   HPLC as an Analytical Technique B.   Instrumental Characteristics C.   GC vs. HPLC:  How Do They Compare?
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II.  The Chromatographic Process in HPLC A.   Stationary Phase 1.   Effect of Particle Size on Separation Efficiency 2.   Stationary Phase Materials   a.   Silica (SiO 2 ) Particles
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b.   Bonded Stationary Phases B.   Adsorption vs. Partition Chromatography
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Unformatted text preview: 1. Adsorption Chromatography 2. Partition Chromatography C. Normal-Phase vs. Reversed-Phase Chromatography 1. Normal-Phase Chromatography 2. Reversed-Phase Chromatography III. The Elution Process in Liquid Chromatography and HPLC A. Isocratic Elution B. The General Elution Problem 1. What is “The General Elution Problem” 2. Solutions to the general elution problem in LC and HPLC C. Eluent Strength ( ˚)...
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This note was uploaded on 11/03/2009 for the course CHEM 241 taught by Professor Tiani during the Summer '08 term at UNC.

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Chapter_25_HPLC_Lecture_Notes - 1. Adsorption...

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