Chromatography_Problem_Set_2

Chromatography_Problem_Set_2 - 6. You inject a sample,...

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Chromatography Problem Set 2 1. Explain why in liquid chromatography it is not feasible to use an open tubular column, but it is feasible in gas chromatography? 2. If you were to use a wall-coated open tubular column, what would be the van Deemter equation? 3. If you were to use a wall-coated open tubular column, which term in the van Deemter equation would be most adversely affected? 4. Draw a block diagram of an HPLC, labeling each of the components. 5. What is the purpose of the guard column? Why is it placed before the actual column instead of after the column?
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Unformatted text preview: 6. You inject a sample, Compound X, onto two different liquid chromatography columns. The first column (Column 1) has 5000 plates whereas the second column has 100 plates. Sketch the chromatographic peak shape of Compound X that would be observed for each of the two columns. How does the peak shape of Compound X, eluted from Column 1, compare to the peak shape of Compound X, eluted from Column 2? 7. Is the separation process in adsorption liquid chromatography any different from the separation process in adsorption HPLC?...
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