Fundamentals_of_Spectrophotometry_Problem_Set_1

Fundamentals_of_Spectrophotometry_Problem_Set_1 - Chemistry...

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Chemistry 41 Spring 2005 Fundamentals of Spectrophotometry Problem Set # 1 1. Define the following terms: frequency, wavenumber, monochromatic light, molar absorptivity and Beer’s law. 2. When does Beer’s law fail and why? 3. Why do we say that light has a dual nature? 4. List the regions of the electromagnetic spectrum in order of decreasing energy. 5. Calculate the absorbance of solutions which have the following percent transmittance (% T) values: (a) 75, (b) 52, (c) 10, (d) 4.1 and (e) 1.0. 6. Convert the following absorbance readings to percent transmittance (% T): (a) 0.005, (b) 0.23, (c) 0.48 (d) 1.00 and (e) 2.0. 7. A solution was found to have a 15.6 % transmittance at its wavelength of maximum absorption using a cell with a path length of 5.00 cm. Calculate the % T and absorbance of the solution in (a) a 10.00 cm and (b) a 1.0 cm cell. 8. A compound has a molar absorptivity of 9,300 M -1 cm -1 at λ = 395 nm. Calculate the molarity of a solution of this compound which has an absorbance of 0.425 when
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