Experiment 2

Experiment 2 - Chris Stanton March 12, 2009 Thurs Lab Rm 41...

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Unformatted text preview: Chris Stanton March 12, 2009 Thurs Lab Rm 41 Experiment 2 Lab Report Experiment 2: The induction of mutations in Esherichia coli by ethyl methane sulfonate and in Salmonella typhimurium by Tn10 Purpose : The purpose of this experiment was to isolate E. coli lac- mutants and S. typhimurium auxotrophs and determine their growth requirements and the differences between the two. Results : Day 2: Table 1 : Observations of E. coli on MacConkey’s Plates Dilution Treated with Control/Mutagen Relative # of colonies % of colonies white (lac- ) 10-5 Control “lawn” in some areas, lots of colonies 10-6 Control Individual colonies 10-5 Mutagen- ethyl methane sulfonate “lawn” in some areas, lots of colonies 4 10-6 Mutagen- ethyl methane sulfonate Individual colonies 8 Day 3: E. coli There were 44 visible white colonies on a plate of 10-5 dilution (approximately 580 total colonies) and 48 white colonies on a plate of 10-6 dilution (approximately 1100 total colonies). The rest of the colonies on the other plates were too close together to be counted individually. S. typhimurium For control: There were no colonies out of the 100 that grew on LB (nutrient agar) and not on the min (minimal agar) plate. For mutagenesis: There were 5 colonies out of 100 that apparently grew on the LB plate and not on the min plate. Day 5: Table 2 : Auxotroph Growth on Different Pool Plates Auxotroph Originally from Control /Mutagen Plate Pool Plates w/ Growth Growth Requirement A Mutagen LB, 2, 7 Leucine B Mutagen LB, all pool plates No Requirement C Mutagen LB, 5, 8 Proline D Mutagen LB, 2, 5, 7, 8 Proline or Leucine E Mutagen...
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This note was uploaded on 11/05/2009 for the course BIO 2810 taught by Professor Fox/nero during the Spring '07 term at Cornell.

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Experiment 2 - Chris Stanton March 12, 2009 Thurs Lab Rm 41...

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