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Unformatted text preview: 5) Set up a master plate from mating F on min + strep. Put 5-10 larger colonies on this master plate.-From the largest colonies, set up master plates from the matings of CSH55 and CSH56 on on min + nal plate. Put 5-10 colonies from mating E and D onto the plate.-Incubate all plates inverted at 37C. 6) Using the sterile toothpicks, patch each colony grown on the master plates to a single plate of min + lactose and a single plate of MacConkeys agar.-Include the control colonies on each plate and incubate plates at 37C. 7) Score the MacConkeys plates 12-20 hours after patching. Score the min + lactose plates 1-2 days after replication. 8) Make sure to record all results....
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This note was uploaded on 11/05/2009 for the course BIO 2810 taught by Professor Fox/nero during the Spring '07 term at Cornell University (Engineering School).
- Spring '07