Experiment 6 - 1 Chris Stanton April 23, 2009 Leah,...

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1 Chris Stanton April 23, 2009 Leah, Thursday Rm 41 Experiment 6: Mitotic Recombination in Saccharomyces cerevisiae Purpose: In this experiment, ade - bakers yeast cells were selected and their phenotypes were examined to determine if the cells became ade - through the process of mitotic recombination. Because mitotic recombination is rare, yeast diploids heterozygous for ARG8 ADE2 HIS3 / arg8 ade2 his3 on chromosome XV and TRP1 / trp1 on chromosome IV were irradiated with UV light before being plated onto several different types of media. A cell that is ade - and has been generated from a mitotic crossover between ade2 and the centromere will have the genotype ARG 8 ade2 his3 / arg8 ade2 his3 while the other cell resulting from this mitosis will be undetectable because it will be a white colony with the genotype ARG8 ADE2 HIS3 / arg8 ADE2 HIS3 . Results: Table 1- Amount of Yeast Growth on Different Media Type of media CSM-His CSM-Ade CSM-Arg CSM-Trp Amount of Growth none none Almost all colonies grew and were red; one did not grow at all All colonies grew and were red Controls: The replica plating on CSM-Ade and CSM-Trp were the controls because the genotype
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This note was uploaded on 11/05/2009 for the course BIO 2810 taught by Professor Fox/nero during the Spring '07 term at Cornell University (Engineering School).

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Experiment 6 - 1 Chris Stanton April 23, 2009 Leah,...

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