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PS3KEY - BMB 100A Fall 2006 Problem Set#3 Name K6 V 1 List...

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Unformatted text preview: BMB 100A Fall, 2006 Problem Set #3 Name K6 V 1. List three properties of the Kornberg DNA polymerase (Pol I) that seemed to Cairns to make it an unlikely enzyme for replication of the genomic DNA. 5,—3’3’ synflusi: can/)I E’e '3’ {chfiaJ—t 3’—> S" meager; 2. Which of Cairns‘s objections are in fact compatible with the known mechanism of DNA replication as we understand it today. Explain. 4” rings 9mm,» a {ea/44w! 05’“ WW - it»! it s'—> 3" €va «plantain My web”! MCRMPCW, nag/3&1!!! aflPfli’M/s 3. The cloning vector pBR322 is a closed, circular double-stranded DNA containing 4.3 kbp of DNA. During transcription, RNA polymerase causes local unwinding of 14 bp of DNA to expose the template strand. If 10 RNA polymerases are transcribing each pBR322 molecule, what linking number is required to prevent any supercoiling during transcription? (You can assume that there are 10 bp per Watson—Crick helical repeat). / N Li‘bofl I“? = £4150 W"- ‘I‘WII’ at ‘-'- “P50 to I: I4 3 Ha bf Una-Inna! .' . W = +H -. "r3 raw Sawtonhi , 1am ma 4. “a...” L "g ’4 r. = ‘i‘So-H = “H; 4. If, starting with a fully base-paired, non—supercoiled pBR322 DNA, topoisomerase I] is used to relax the supercoiling caused by the transcription in problem , how many ATPs would be consumed? 3 3'! Romp: MUN-EV; SVWMHS hum-z 73"1- -‘ ’M header—java! \- T.?.JT uh, 2 our 2.x '4 r(i‘{ Ann. 5. What are the functions of the following in DNA replication? DNAligase Jain”? 0kfiuki (Pip-“1-s 3‘-exonuclease of DNA polymerase III T“? of -: M4431}? DNA gyrasc reig%o Jamal/{n SSB my: 5 .5. vie/w; Lute 5% ages/f Mme {l/flth‘Fdr‘f {CA/A yr/M-(fnfijbmlj [3 subunit of DNA polymerase III fro cessi v H7 DNA polmerasel MPflir + Pr'w mmwfii Okazaki fragments #77 DJ 5 firm J 9.,” 119m- 6. Why must eukaryotic chromosomes end in telomeres? annuwm, (kt-l wmloi 5M4“. a each WA 99' MHC‘J-lml Whai align (m Jo Fwd-L9 .34 1mm) La ...
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