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Unformatted text preview: forms. 5. Draw the Watson-Crick base pairs A-T and G-C (show only the bases, and the positions of the glycosyl bonds). 6. Draw the mononucleotide 7-methyl-guanosine-5'-monophosphate. Show all formal charges. 7. Draw the ionic forms of cytosine that would exist in solution at pH 4.5. Predict the relative proportions of each that would be present. 8. The four nucleoside monophosphates can be separated from each other by electrophoresis at pH 3.5. (Electrophoresis means separation of charged molecules in an electric field based on differing charges for the different molecules.) Predict the relative mobilities of the four nucleotides with respect to the cathode and anode, starting at a position equidistant from the cathode and anode. Explain why pH 3.5 is commonly used for this separation....
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This note was uploaded on 11/07/2009 for the course BIOCHEM bIO taught by Professor Professor during the Spring '09 term at École Normale Supérieure.
- Spring '09