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Practice Final Exam

Practice Final Exam - Practice Final Exam Name Use the...

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Practice Final Exam Name:_________________________ Use the following list of enzymes to answer questions # 1-4. Each answer can be used more than once. a). helicase, b). DNA polymerase, c). ligase, d). primase, e). RNA polymerase 1. Catalyzes the synthesis of a new strand of DNA. 2. Enhances DNA strand separation during replication. 3. Covalently connects segments of DNA. 4. Synthesizes a short RNA molecule during DNA replication. Use the following to answer questions # 5-9. Answers can be used more than once. a). Hershey and Chase, b). Chargaff's rules, c). Watson and Crick, d). Meselson and Stahl, e). none of the above 5. This/these investigator(s) found that [pyrimidines] = [purines]. 6. The model for B form DNA was described by _______. 7. ________ developed a vaccine for bacterial pneumonia. 8. That DNA is the transforming factor for bacteriophage was determined by _______. 9. If cytosine makes up 22% of the nucleotides in a sample of DNA from an organisms, then adenine would make up what percent of the bases? a). 22%, b). 44%, c). 28%, d). 56%, e). can not be determined from the information provided. 10. The problem of replicating the lagging strand -- that is, adding bases in apparently the 3' to 5' direction -- is solved by DNA through the use of a). base pairing, b). replication forks, c). the unwinding enzyme, helicase, d). Okazaki fragments, e). topoisomerase 11. When T2 phage viruses that infected bacteria make more viruses in the presence of radioactive sulfur, which of the following results? a. the viral DNA is tagged by radioactivity b. the viral proteins are tagged by radioactivity c. the viral DNA is found to be of medium density in a centrifuge tube d. they transfer their radioactivity to E. coli DNA e. both viral DNA and viral proteins are tagged by radioactivity 12. Suppose one were provided with an actively dividing culture of E. coli bacteria to which radioactive thymine had been added. What would happen is a cell replicated once in the presence of this radioactive base? (Hint: think semiconservative DNA replication) a. one of the daughter cells, but not the other would have radioactive DNA b. neither of the 2 daughter cells would be radioactive c. all 4 bases of the DNA would be radioactive d. radioactive thymine would pair with nonradioactive guanine e. DNA in both daughter cells would be radioactive
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13. In the Meselson-Stahl experiment, bacteria were grown in medium containing 15 N and were then transferred to a medium containing 14 N. Which of the results would be expectedafter 2 semiconservative DNA replication in 14 N? 14. It became apparent to Watson and Crick after completion of their model that the DNA molecule could carry a vast amount of hereditary information in its a). sequence of bases, b). phosphate-sugar backbones, c). complementary base pairing, d). side groups of nitrogenous bases, e). different 5 carbon sugars 15. What is required for the catalytic activity of DNA polymerase?
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