Lecture 3 yeast mutation

Lecture 3 yeast mutation - UV Mutagenesis in Yeast...

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UV Mutagenesis in Yeast Geneticists need variation to study the function of gene products. We create variation in the laboratory by mutagenesis
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Features of a good model organism Ease of Genetic Manipulation Biochemistry Cell biology Conserved cellular   processes DNA proteins 1 2 3 5 6 4
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Important lab properties Genetics: introduce mutations (UV, chemical, Xray) and design screens to identify mutations in process you are interested in Isolate the products of meiosis Recover mutations: stable haploid and diploid lifecycles. Easy molecular manipulations-clonability (few introns), high rate of homologous recombination
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Fig. 7.2 Mutations are classified by what they do to DNA
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Fig. 7.6
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Fig. 7.12b1
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Fig. 7.12b2
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By choosing the correct mutagens, we can control the type of mutations we make. Because most of the chemicals are nasty, you will be using UV light to generate mutations
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Formation of Thymidine Dimer sugar sugar sugar sugar
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This note was uploaded on 11/08/2009 for the course BIOL 423l taught by Professor Staff during the Fall '08 term at UNC.

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Lecture 3 yeast mutation - UV Mutagenesis in Yeast...

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