Molecular Genetics 6

Molecular Genetics 6 - Molecualr Genetics V1 Nov.4,2008...

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Molecualr Genetics V1 Nov.4,2008 Francke 6 1 11/3/08 HumBio2A Molec.Genetics Vi 1 1. What is biotechnology? 2. Recombinant DNA methods (cloning DNA) 3. Using recombinant DNA to make therapeutic proteins 4. Genetically modified crops (food) 5. Manipulating genes in mice 6. Induced pluripotent stem cells to cure diseases Molecular Genetics VI Recombinant DNA and Biotechnology 11/3/08 HumBio2A Molec.Genetics Vi 2 Biotechnology A few of the many applications: • Producing protein-based drugs, hormones, vaccines, other useful proteins • Forensics, human genetic diagnostics • Agricultural improvement of crops • Converting organic waste to less harmful products • Cleanup of environmental damage The use of organisms (bacteria, yeast, cultured cells, even whole organisms) or substances made from them (like enzymes) to perform industrial, manufacturing, agricultural or environmental processes 11/3/08 HumBio2A Molec.Genetics Vi 3 Recombinant DNA technology DNA cloning First developed by Stanley Cohen of Stanford and Herbert Boyer of UCSF in the 1973 Each was working on basic research problems that did not initially seem to have practical uses Their work is the basis of the biotechnology industry and academic research laboratories that use DNA cloning Stanley Cohen Dept. Genetics Stanford University School of Medicine Herbert Boyer Dept. Biochemistry, UCSF Founder of Genentech
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Molecualr Genetics V1 Nov.4,2008 Francke 6 2 11/3/08 HumBio2A Molec.Genetics Vi 4 Three important components of DNA cloning 1. Restriction enzymes 2. DNA ligase 3. Cloning vector 11/3/08 HumBio2A Molec.Genetics Vi 5 Restriction enzymes ( restriction endonucleases ) cut double-stranded DNA into smaller pieces. Bacteria use these as defense against DNA from bacteriophage Restriction enzyme cuts Phage DNA Other enzymes degrade it further Bacterial DNA protected by methylation at cleavage sites Life 8 . Chapter 16 11/3/08 HumBio2A Molec.Genetics Vi 6 Restriction enzymes cut DNA at specific
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This note was uploaded on 11/08/2009 for the course HUMBIO 2A taught by Professor Boggs,c;durham,w during the Spring '08 term at Stanford.

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Molecular Genetics 6 - Molecualr Genetics V1 Nov.4,2008...

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