Molecular Genetics 7

Molecular Genetics 7 - Molecular Genetics VII Nov.5,2008...

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Molecular Genetics VII Nov.5,2008 Francke 7 1 11/4/08 HumBio2A: Molec.Genetics VII 1 Why and how was it sequenced? What are the characteristics of • the human genome • and human genes What is the role of noncoding RNA ? Molecular Genetics VII The Human Genome 11/4/08 HumBio2A: Molec.Genetics VII 2 The 2000 Draft of the Human Genome Sequence Announced in June 2000 Published in Feb 2001 Venter and 282 oauthors International Human Genome Sequencing Consortium (20 Genome Centers in 6 countries) HumBio2A: Molec.Genetics VII 3 Genome Centers around the World In the Limelight
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Molecular Genetics VII Nov.5,2008 Francke 7 2 11/4/08 HumBio2A: Molec.Genetics VII 4 Robotics Sequencing Instruments Computers Informatics 1996 1997 1998 1999 2000 FINISHED UNFINISHED (DRAFT) HUMAN GENOME SEQUENCE IN DATABASES 4,500 Mb Cloning large DNA fragments: Yeast artificial chromosomes (YAC) (~250,000 bp inserts) Bacterial artificial chromosomes (BAC) (~120,000 bp inserts) YAC vector 11/4/08 HumBio2A: Molec.Genetics VII 6 How the mapping of the /YAC/BAC clones is done Recombinant DNA: Genes and Genomes. 3rd Edition (Dec06). WH Freeman Press. Human genomic YAC and BAC libraries were essential for mapping and sequencing the human genome
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Molecular Genetics VII Nov.5,2008 Francke 7 3 11/4/08 HumBio2A: Molec.Genetics VII 7 Hierarchical versus shotgun sequencing Start with mapped/aligned BACs Start with fragments of whole genome International Consortium Celera company 11/4/08 HumBio2A: Molec.Genetics VII 8 2003: complete sequence of the human genome 11/4/08 9 The Number of Human Genes N ~ 20,000 Only 2,000 more than the worm Only 7,000 more than the fruitfly
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Molecular Genetics VII Nov.5,2008 Francke 7 4 11/4/08 HumBio2A: Molec.Genetics VII 10 Complexity Issues 100 trillion cells >300 cell types 3 billion letters in genome in 46 chromosomes
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Molecular Genetics 7 - Molecular Genetics VII Nov.5,2008...

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