Quiz 3 WED key - BIO325 Genetics Instructor Dr Tracey...

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BIO325 Genetics Instructor: Dr. Tracey Ruhlman TA: Teofil Nakov QUIZ 3 Points are given in parentheses after each question. Total points = 20. 1. True or False: (1 point each) a) DNA polymerase III in E. coli is responsible for replication of both the leading and the lagging strand. TRUE b) DNA polymerase I is a processive enzyme. FALSE c) Apurinic sites are created by removal of a nucleotide containing G or A. FALSE d) The normal tautometic form of nucleotides is their keto form. TRUE 2. The enzyme that adds repetitive sequences, called telomeres to the ends of chromosomes in eukaryotes (prokaryotes/eukaryotes) is called telomerase. This enzyme uses a RNA ( RNA/DNA) template for synthesis of DNA (RNA/DNA). Therefore this enzyme has activity of a reverse transcriptase (DNA polymerase/RNA polymerase/reverse transcriptase). During this process the template (template/newly synthesized) strand is being elongated. (1 point each blank)
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3. In mismatch repair in E. coli , only a mismatch in the newly synthesized strand is corrected. How is
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