Quiz 4 KEY - BIO325 Genetics Instructor: Dr. Tracey Ruhlman...

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BIO325 Genetics Instructor: Dr. Tracey Ruhlman TA: Teofil Nakov QUIZ 4 Points are given in parentheses after each question. Total points = 20. 1. The following is a diagram of integration of a transposon into a new site in the genome. Label the four steps indicated in the diagram stating what if any enzymes are involved in each step. (1 point label) 1 = Transposase recognizes insertion site and makes staggered cut 2 = Transposon DNA inserted into new site and is ligated to overhangs of sticky ends 3 = DNA polymerase I fills in the gaps left at the overhangs 4. Direct repeats are formed, and ligase joins newly synthesized DNA. 2. Match the types of transposons to features of transposons. (1 point each) 1. Composite = C A. Uses RNA intermediate 2. Simple = E B. Cut and paste transposition 3. Conservative = B C. Transposase coded in flanking Inverted Repeats (IR) 4. Replicative = F D. Autonomous retrotransposons in the human genome 5. Retrotransposon = A E. Transposase coded in transposon itself (not IR's) 6. LINE = D F. Copy and paste transposition
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3. Compare and contrast (Briefly!) the following pairs of terms regarding large scale
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This note was uploaded on 11/11/2009 for the course BIO 325 taught by Professor Saxena during the Spring '08 term at University of Texas at Austin.

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Quiz 4 KEY - BIO325 Genetics Instructor: Dr. Tracey Ruhlman...

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