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Quiz 5 2009 WED - KEY

Quiz 5 2009 WED - KEY - phosphorylation and...

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BIO325 Genetics Instructor: Dr. Tracey Ruhlman TA: Teofil Nakov QUIZ 5 Points are given in parentheses after each question. Total points = 20. 1. This diagram represents regions around a gene encoding mRNA. The letters A-F above the diagram refer to areas of the gene and its transcript located directly below them. AUG is the start codon of the future protein and UGA is the stop codon for the future protein. (1 point each) a) What region of the DNA will be bound by TFIID+TBP A . b) Where will transcription start B . c) The region between E and F is called 3' UTR . d) What region of the primary transcript is removed by the spliceosome D . 2. True or false about mRNA processing and RNA interference in eukaryotes. (1 pt. each) a) The 7methylguanosine cap is added on the 3' end of mRNA molecules. FALSE b) The RISC complex contains a short single stranded RNA molecule used to find and degrade unwanted RNA species in the cell. TRUE c) The function of the Carboxyl Terminal Domain of RNA pol II is controlled through
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Unformatted text preview: phosphorylation and dephophorylation. TRUE d) The polyadenine tail of mRNA molecules is added because genes end with a stretch of few hundred thymines. FALSE 3. The following diagrams depict three stages in the functioning of the lac operon. State what should be the levels of lactose and glucose in the media in order to observe each stage. (2 points each) A= glucose present; NO lactose; B= glucose present, lactose present; C= NO glucose, lactose present 4. Fill in the blanks (2 points each): a) In splicing, the first transesterification reaction involves the 5' end of the intron and the branch site A of the intron forming a lariat. In the second transesterification the phosphodiester bond between the intron and exon 2 is cut by the free OH at the 3' end of exon 1 thereby releasing the intron and ligating the two exons. The molecular machine (spliceosome) helping this process is a complex of proteins and snRNA's . ( a diagram is presented below.)...
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